منابع مشابه
Reporter gene fusions.
Copyright: © 2006 Thomas Boulin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Both of these authors contributed equally To whom correspondence should be addressed. E-mail: [email protected] Reporter g...
متن کاملRapid engineering of bacterial reporter gene fusions by using Red recombination.
Reporter gene fusions are essential tools for the investigation of gene regulation. Such fusions are traditionally generated by transposon mutagenesis and identified by a suitable selection procedure. Alternatively, specific reporter fusions can be generated by cloning of DNA fragments containing promoters or other regulatory elements in reporter plasmids. Here, we describe a novel approach for...
متن کاملDominant positive and negative selection using luciferase, green fluorescent protein and beta-galactosidase reporter gene fusions.
The introduction of exogenous DNA sequences into cultured cells is a cornerstone in the study of mammalian gene expression. For most cell types, the efficiency of stable gene transfer is usually quite low, which mandates the use of selectable markers to isolate a population of transfected cells with the desired phenotype. In simple gene expression experiments, only one type of selectable marker...
متن کاملA New Reporter Gene Technology: Opportunities and Perspectives
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
متن کاملDetecting and visualizing gene fusions.
In recent years, gene fusions have gained significant recognition as biomarkers. They can assist treatment decisions, are seldom found in normal tissue and are detectable through Next-generation sequencing (NGS) of the transcriptome (RNA-seq). To transform the data provided by the sequencer into robust gene fusion detection several analysis steps are needed. Usually the first step is to map the...
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ژورنال
عنوان ژورنال: WormBook
سال: 2006
ISSN: 1551-8507
DOI: 10.1895/wormbook.1.106.1